DRG 25-OH-Vitamin D ELISA (EIA-5396)

Reliable and fast way to discover Vitamin D

The DRG 25-OH Vitamin D (total) ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of total 25-OH Vitamin D (Vitamin D2 / D3) in serum or plasma.

25-OH-Vitamin D (total) assay characteristics:

  • Assay Principle: Competitive ELISA
  • Dynamic range: 3.22 – 120 ng/mL
  • Total assay time: less than 2 h (60/30/15 minutes)
  • Sample volume: 50 µL Serum or Plasma (EDTA, lithium heparin or citrate plasma)
  • Mean Intra Assay precision: 3.7 %
  • Mean Inter Assay precision: 7.13 %
  • Specificity: 99.5% of 25-OH-Vitamin D3 and 97.4% of 25-OH-Vitamin D2

Clinical Benefit:

Vitamin D is a pro-hormone that has long been known for its important role in regulating bone mineralization. Moreover, in recent years, Vitamin D has become an assay of general health status, and there have been multiple publications linking Vitamin D deficiency to several disease states, such as cancer, cardiovascular disease, diabetes and autoimmune diseases.

Advantages of the DRG ELISA (EIA-5396):

  • Easy and straight forward assay procedure without any sample pre-treatment
  • Measurement of 25-OH Vitamin D2 and D3
  • First results in less than 2 hours
  • Standards are calibrated against reference material DEQAS No. 548
  • Negligible cross-reaction to 3-epi-25-OH Vitamin D
  • Reagents are ready-to-use, 2 controls included in the kit
  • Excellent correlation to competition (e.g. Diasorin LIAISON)

Consequences of Vitamin D deficiency:

Bone Metabolism

  • Osteoporosis
  • Osteomalacia
  • Rickets


  • Possibly associated with different cancers (breast, colon, prostate, ovarian)

Cardiac Diseases

  • Stroke
  • High blood pressure
  • Increasing risk for all-cause and cardiovascular mortality

Autoimmune Diseases

  • Diabetes mellitus Type 1
  • Multiple Sclerosis
  • Rheumatoid Arthritis